Ikeda M, Dewar D, McCulloch J
Wellcome Surgical Institute & Hugh Fraser Neuroscience Laboratories, University of Glasgow, Scotland, United Kingdom.
J Neural Transm Park Dis Dement Sect. 1993;5(3):177-84. doi: 10.1007/BF02257672.
ATP-sensitive K+ channels were examined in sections of the hippocampus from patients with Alzheimer's disease and age-matched control subjects by means of quantitative autoradiography. ATP-sensitive K+ channels were labelled with the sulfonylurea, [3H]-glibenclamide, which is a potent blocker of these channels. The density of cells in the subiculum and the activity of choline acetyltransferase were determined in the same hippocampal tissue samples. In the hippocampal formation of control subjects, the density of high affinity [3H]-glibenclamide binding sites ranged from 17.6 +/- 0.9 pmoles/g in the presubiculum to 11.6 +/- 0.6 pmoles/g in the parvo-pyramidal layer of the presubiculum. There was no difference between Alzheimer patients and controls in the level of high affinity [3H]-glibenclamide binding in any hippocampal region although there was a marked loss of subicular cells (reduced by 29% compared to controls) and a reduction in choline acetyltransferase activity (reduced by 60% compared to controls). The results suggest that ATP-sensitive K+ channels are associated with elements in the hippocampus which are preserved in Alzheimer's disease.
通过定量放射自显影法，对阿尔茨海默病患者及年龄匹配的对照者海马切片中的ATP敏感性钾通道进行了检测。ATP敏感性钾通道用磺脲类药物[3H] - 格列本脲标记，该药物是这些通道的有效阻滞剂。在相同的海马组织样本中测定了下托中的细胞密度和胆碱乙酰转移酶的活性。在对照者的海马结构中，高亲和力[3H] - 格列本脲结合位点的密度在海马前下托中为17.6±0.9皮摩尔/克，在海马前下托的小锥体细胞层中为11.6±0.6皮摩尔/克。尽管下托细胞明显减少（与对照组相比减少了29%）且胆碱乙酰转移酶活性降低（与对照组相比降低了60%），但在任何海马区域，阿尔茨海默病患者与对照组的高亲和力[3H] - 格列本脲结合水平均无差异。结果表明，ATP敏感性钾通道与海马中在阿尔茨海默病中得以保留的成分相关。
