Ikeda M, Dewar D, McCulloch J
Wellcome Surgical Institute, University of Glasgow, U.K.
Brain Res. 1991 Dec 24;568(1-2):303-6. doi: 10.1016/0006-8993(91)91414-v.
[125I]Galanin binding sites were examined in the hippocampal region of patients with Alzheimer's disease (AD) and age-matched control subjects using quantitative autoradiography. In control subjects, [125I]galanin binding sites were highly concentrated in the presubicular parvopyramidal layer (5.60 +/- 0.74 pmol/g), while other hippocampal regions had considerably lower levels of binding (0.56-1.73 pmol/g). In the majority of hippocampal regions, [125I]galanin binding was similar in AD patients to that in controls with a significant reduction being observed only in the deep layers of the parahippocampal gyrus. The activity of choline acetyltransferase, determined in the same tissue samples used for autoradiographic studies, was markedly reduced in AD (controls 6.8 +/- 0.6; AD 2.7 +/- 0.6 nmol/h/mg protein, mean +/- S.E.M.). These data are not consistent with a presynaptic location on cholinergic terminals of galanin binding sites in the human hippocampus unless upregulation has occurred.
使用定量放射自显影技术，对阿尔茨海默病（AD）患者和年龄匹配的对照受试者的海马区中[125I]甘丙肽结合位点进行了检测。在对照受试者中，[125I]甘丙肽结合位点高度集中在海马旁下托小锥体细胞层（5.60±0.74 pmol/g），而海马其他区域的结合水平则低得多（0.56 - 1.73 pmol/g）。在大多数海马区域，AD患者的[125I]甘丙肽结合情况与对照组相似，仅在海马旁回深层观察到显著减少。在用于放射自显影研究的相同组织样本中测定的胆碱乙酰转移酶活性，在AD患者中显著降低（对照组6.8±0.6；AD组2.7±0.6 nmol/h/mg蛋白，平均值±标准误）。除非发生上调，否则这些数据与人脑海马区甘丙肽结合位点位于胆碱能终末突触前的位置不一致。
