Konopacki J, Gołebiewski H
Department of Neurobiology, University of Lódz, Poland.
Neuroreport. 1993 Jul;4(7):963-6. doi: 10.1097/00001756-199307000-00032.
Brain slice preparations obtained from the rat were used to study cholinergic GABAergic interaction in mechanisms responsible for production of theta-like activity in hippocampal slices maintained in vitro. Bicuculline, a GABA-A antagonist, applied at 25 microM facilitated the effect of low concentration carbachol (25 microM) in inducing theta-like oscillations. At 100 microM, bicuculline increased the amplitude of carbachol-induced theta-like slow waves. This carbachol-bicuculline induced field potential was antagonized by a muscarinic blocker, atropine sulphate, and a GABA-A agonist, muscimol. These results provide in vitro evidence for cholinergic-GABAergic interaction in the production of hippocampal theta-like slow waves.
从大鼠获取的脑片标本用于研究胆碱能与γ-氨基丁酸能（GABAergic）在体外维持的海马脑片中产生θ样活动机制中的相互作用。荷包牡丹碱，一种GABA-A拮抗剂，以25微摩尔的浓度应用时，可促进低浓度卡巴胆碱（25微摩尔）诱导θ样振荡的作用。在100微摩尔时，荷包牡丹碱增加了卡巴胆碱诱导的θ样慢波的幅度。这种卡巴胆碱-荷包牡丹碱诱导的场电位被一种毒蕈碱阻滞剂硫酸阿托品和一种GABA-A激动剂蝇蕈醇所拮抗。这些结果为海马θ样慢波产生过程中胆碱能与GABA能的相互作用提供了体外证据。
