Kowalczyk Tomasz, Konopacki Jan
Department of Neurobiology, University of Lódź, Lódź, Poland.
Brain Res Bull. 2002 Sep 30;58(6):569-74. doi: 10.1016/s0361-9230(02)00827-4.
Phase and amplitude depth profiles of EEG theta-like activity (TLA) induced by bath perfusion of 50 micro M of the cholinergic agonist carbachol were investigated using rat hippocampal formation slices. The data showed that the amplitude and phase of laminar profiles of TLA recorded in vitro were the same as the type III theta profiles recorded in in vivo rat preparations. These results supported the conclusion that the neural mechanisms involved in the generation of theta field activity in in vivo preparations and the generation of TLA in in vitro preparations were similar. The study provided further validation for the use of carbachol in vitro slice preparation as a model for studying the neural circuitry underlying theta generation in the hippocampal formation.
使用大鼠海马结构切片，研究了用50微摩尔胆碱能激动剂卡巴胆碱进行浴灌流诱导的脑电图类θ活动（TLA）的相位和幅度深度分布。数据表明，体外记录的TLA层状分布的幅度和相位与体内大鼠制备中记录的III型θ分布相同。这些结果支持了以下结论：体内制备中θ场活动产生和体外制备中TLA产生所涉及的神经机制相似。该研究为在体外切片制备中使用卡巴胆碱作为研究海马结构中θ产生潜在神经回路的模型提供了进一步验证。
