家蝇（Musca domestica）唾液腺增生相关病毒的特性鉴定与描述

Characterization and description of a virus causing salivary gland hyperplasia in the housefly, Musca domestica.

作者信息

Coler R R, Boucias D G, Frank J H, Maruniak J E, Garcia-Canedo A, Pendland J C

机构信息

Department of Entomology and Nematology, University of Florida, Gainesville 32611-0620.

出版信息

Med Vet Entomol. 1993 Jul;7(3):275-82. doi: 10.1111/j.1365-2915.1993.tb00688.x.

DOI:10.1111/j.1365-2915.1993.tb00688.x

PMID:8369563

Abstract

A double-stranded DNA virus was isolated from hyperplasic salivary glands of male and female houseflies, Musca domestica L. (Diptera: Muscidae), collected from a dairy in Alachua County, Florida, U.S.A. Sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE) of this housefly salivary gland hyperplasia (SGH) virus revealed the presence of two major and eight minor structural polypeptides. Restriction endonuclease analysis indicated that the c. 137 kilobase pair DNA was double-stranded. Weekly, sweep-net sampling of the fly population throughout the season (May-October, 1991) showed that 1.5-18.5% of the dissected flies possessed hyperplasic salivary glands. The virus replicated within the nuclei of the salivary gland cells and was transmitted per os to newly-emerged healthy adult flies.

摘要

从美国佛罗里达州阿拉楚阿县一家奶牛场采集的家蝇（双翅目：蝇科）雌雄个体的增生唾液腺中分离出一种双链DNA病毒。对这种家蝇唾液腺增生（SGH）病毒进行十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳（PAGE）分析，结果显示存在两种主要结构多肽和八种次要结构多肽。限制性内切酶分析表明，约137千碱基对的DNA是双链的。在整个季节（1991年5月至10月）每周对蝇群进行扫网采样，结果显示，在解剖的苍蝇中，1.5%至18.5%的个体拥有增生的唾液腺。该病毒在唾液腺细胞核内复制，并通过口传播给新羽化的健康成年苍蝇。