Pannekoek Y, Schuurman I G, Dankert J, van Putten J P
Department of Medical Microbiology, University of Amsterdam, The Netherlands.
Clin Exp Immunol. 1993 Sep;93(3):377-81. doi: 10.1111/j.1365-2249.1993.tb08188.x.
Acute- and convalescent-phase sera from 40 patients with meningococcal disease were evaluated for immunoreactivity with the meningococcal member of the hsp60 stress protein family. The IgG response was measured by ELISA, using bacterial cell lysate of the corresponding patients' strain, and purified hsp60 proteins from Neisseria meningitidis (MSP63), Escherichia coli (GroEL) and Mycobacterium bovis BCG (65K) as antigens. Analysis of the antibody responses revealed that 24/35 patients (69%) with elevated anti-meningococcal titres, generated anti-MSP63 antibodies during the time course of infection. Twelve of these patients generated antibodies specific for MSP63, in six patients anti-MSP63 levels exceeded anti-GroEL/65K antibodies. In the remaining six patients, equal levels of anti-MSP63 and anti-GroEL/65K were measured. We conclude that MSP63 is expressed and immunogenic during natural meningococcal infection, and that individual subjects have a restricted response to the antigen, resulting in the recognition of Neisseria-specific hsp60 epitopes and/or cross-reactive hsp60 determinants.
对40例脑膜炎球菌病患者的急性期和恢复期血清进行评估，以检测其与热休克蛋白60（hsp60）应激蛋白家族中脑膜炎球菌成员的免疫反应性。采用酶联免疫吸附测定（ELISA）法，以相应患者菌株的细菌细胞裂解物以及从脑膜炎奈瑟菌（MSP63）、大肠杆菌（GroEL）和牛分枝杆菌卡介苗（65K）中纯化的hsp60蛋白作为抗原，检测IgG反应。抗体反应分析显示，在35例抗脑膜炎球菌滴度升高的患者中，有24例（69%）在感染过程中产生了抗MSP63抗体。其中12例患者产生了针对MSP63的特异性抗体，6例患者的抗MSP63水平超过了抗GroEL/65K抗体。在其余6例患者中，抗MSP63和抗GroEL/65K的水平相当。我们得出结论，在自然脑膜炎球菌感染期间，MSP63具有表达且具有免疫原性，并且个体对该抗原的反应有限，从而导致对奈瑟菌特异性hsp60表位和/或交叉反应性hsp60决定簇的识别。
