Wagar E A, Schachter J, Bavoil P, Stephens R S
Department of Laboratory Medicine, University of California, San Francisco.
J Infect Dis. 1990 Oct;162(4):922-7. doi: 10.1093/infdis/162.4.922.
Chlamydia trachomatis causes sexually transmitted diseases and is associated with serious long-term sequelae such as tubal infertility and ectopic pregnancy. There have been suggestions that chlamydial antigens of approximately 57,000-60,000 Mr may be involved in the immunopathology. Two important chlamydial antigens of 57,000-60,000 Mr are a Triton X-100-soluble antigen, which induces hypersensitivity in ocular models, and a sarcosyl-insoluble cysteine-rich structural protein, omp2. In this study, a 57,000 Mr Triton X-100-soluble protein was characterized as the chlamydial homolog of groEL, a heat shock protein. Using protein fractions, antibody responses of pelvic inflammatory disease (PID) and ectopic pregnancy patients to chlamydial groEL and omp2 were differentiated. Nearly all patients in both groups were reactive to omp2. Of those with titers greater than or equal to 1:512, 31% of PID sera and 81% of ectopic pregnancy sera were positive for chlamydial groEL (P = .004). This selectivity suggests that women with PID who develop chronic sequelae are those with antibody to groEL.
沙眼衣原体可引发性传播疾病，并与诸如输卵管性不孕和异位妊娠等严重的长期后遗症相关。有观点认为，分子量约为57,000 - 60,000的衣原体抗原可能参与免疫病理学过程。两种分子量为57,000 - 60,000的重要衣原体抗原，一种是Triton X - 100可溶性抗原，可在眼部模型中诱导超敏反应，另一种是肌氨酸不溶性富含半胱氨酸的结构蛋白omp2。在本研究中，一种分子量为57,000的Triton X - 100可溶性蛋白被鉴定为热休克蛋白groEL的衣原体同源物。利用蛋白质组分，区分了盆腔炎（PID）和异位妊娠患者对衣原体groEL和omp2的抗体反应。两组中几乎所有患者对omp2均有反应。在滴度大于或等于1:512的患者中，31%的PID血清和81%的异位妊娠血清衣原体groEL呈阳性（P = 0.004）。这种选择性表明，出现慢性后遗症的PID女性是那些具有groEL抗体的女性。
