Gabrielli A, Di Loreto C, Taborro R, Candela M, Sambo P, Nitti C, Danieli M G, DeLustro F, Dasch J R, Danieli G
Istituto di Clinica Medica, Università di Ancona, Italy.
Clin Immunol Immunopathol. 1993 Sep;68(3):340-9. doi: 10.1006/clin.1993.1136.
We have investigated the distribution of TGF beta using antibodies specific for its intracellular and extracellular forms in full-thickness biopsies of patients with SSc, primary Raynaud's phenomenon (PRP), systemic lupus erythematosus (SLE), and from normal subjects. Nine of 11 SSc biopsies demonstrated intracellular TGF beta in endothelial cells while only 6 exhibited extracellular TGF beta. Endothelial cells in skin biopsies of all PRP patients displayed both intracellular and extracellular TGF beta. All other control biopsies were negative. In patients with PRP, some positively staining fibroblasts were found scattered throughout the dermis. Lastly, extracellular TGF beta was localized in the papillary dermis of PRP and SSc biopsies and in all the dermal layers of SLE patients. No significant staining of TGF beta was observed in the endothelial cells, fibroblasts, or in the extracellular matrix of the majority of biopsies from normal subjects. These data suggest that TGF beta may be one of the cytokines involved in the early stages of pathogenesis of SSc, and that endothelial cells in SSc and PRP may be a source and/or a target of TGF beta.
我们使用针对转化生长因子β（TGFβ）细胞内和细胞外形式的特异性抗体，对系统性硬化症（SSc）、原发性雷诺现象（PRP）、系统性红斑狼疮（SLE）患者以及正常受试者的全层活检组织进行了TGFβ分布情况的研究。11例SSc活检组织中有9例在内皮细胞中显示出细胞内TGFβ，而只有6例显示出细胞外TGFβ。所有PRP患者皮肤活检中的内皮细胞均同时显示出细胞内和细胞外TGFβ。所有其他对照活检均为阴性。在PRP患者中，发现一些阳性染色的成纤维细胞散在于整个真皮层。最后，细胞外TGFβ定位于PRP和SSc活检组织的乳头真皮层以及SLE患者的所有真皮层。在大多数正常受试者的活检组织中，未在内皮细胞、成纤维细胞或细胞外基质中观察到TGFβ的显著染色。这些数据表明，TGFβ可能是参与SSc发病早期阶段的细胞因子之一，并且SSc和PRP中的内皮细胞可能是TGFβ的来源和/或靶点。
