Marshall J S, Gauldie J, Nielsen L, Bienenstock J
Molecular Virology and Immunology Programme, McMaster University, Hamilton, Ontario, Canada.
Eur J Immunol. 1993 Sep;23(9):2116-20. doi: 10.1002/eji.1830230911.
Leukemia inhibitory factor (LIF) is a pluripotent cytokine of importance in the regulation of immune and inflammatory responses. This cytokine may play an important role in neuronal development and bone metabolism. We have examined the ability of freshly isolated rat mast cells and mast cell lines to produce LIF at both the mRNA and bioactivity levels. Initial experiments demonstrated that two mucosal mast cell-like cell lines RBL.2H3 and RCMC9 endogenously produced low levels of LIF bioactivity. The production of this cytokine was examined using a hepatocyte-stimulating factor activity assay and confirmed by the use of neutralizing antibodies specific for LIF. This production was enhanced by treatment with the calcium ionophore A23187. No interleukin-6 production was observed by these cells either endogenously or following ionophore activation. Freshly isolated highly purified rat peritoneal mast cells also expressed mRNA for LIF. These results could have important implications for the role of mast cells in neuronal development, hematopoiesis bone metabolism and the acute-phase response.
白血病抑制因子（LIF）是一种在免疫和炎症反应调节中具有重要作用的多能细胞因子。这种细胞因子可能在神经元发育和骨代谢中发挥重要作用。我们已经检测了新鲜分离的大鼠肥大细胞和肥大细胞系在mRNA和生物活性水平上产生LIF的能力。初步实验表明，两种黏膜肥大细胞样细胞系RBL.2H3和RCMC9内源性产生低水平的LIF生物活性。使用肝细胞刺激因子活性测定法检测了这种细胞因子的产生，并通过使用针对LIF的中和抗体进行了确认。用钙离子载体A23187处理可增强这种产生。这些细胞无论是内源性还是在离子载体激活后均未观察到白细胞介素-6的产生。新鲜分离的高度纯化的大鼠腹膜肥大细胞也表达LIF的mRNA。这些结果可能对肥大细胞在神经元发育、造血、骨代谢和急性期反应中的作用具有重要意义。
