Kamohara H, Sakamoto K, Ishiko T, Mita S, Masuda Y, Abe T, Ogawa M
Department of Surgery II, Kumamoto University Medical School, Japan.
Res Commun Mol Pathol Pharmacol. 1994 Aug;85(2):131-40.
We investigated the production of leukemia inhibitory factor (LIF) by human carcinoma cell lines. LIF mRNA was detected by Northern blot analysis in all 24 carcinoma cell lines of the lung, breast, stomach, colon, liver, gallbladder, pancreas and melanocytes. Seventeen of them (70.8%) secreted LIF in the culture supernatant (range: 40.4-3990.3 pg/ml, mean +/- SEM: 611.8 +/- 262.9 pg/ml). Biologic activity of LIF was confirmed in the culture supernatant of carcinoma cell lines by the MTT assay using M1 cells. The present results showed that human carcinoma cell lines are constitutively producing biologically active LIF. The possible biological significance of LIF produced by cancer cells is discussed.
我们研究了人癌细胞系中白血病抑制因子（LIF）的产生情况。通过Northern印迹分析在所有24种肺、乳腺、胃、结肠、肝、胆囊、胰腺癌细胞系及黑色素细胞系中检测到了LIF mRNA。其中17种（70.8%）在培养上清液中分泌LIF（范围：40.4 - 3990.3 pg/ml，平均±标准误：611.8 ± 262.9 pg/ml）。使用M1细胞通过MTT试验在癌细胞系的培养上清液中证实了LIF的生物活性。目前的结果表明人癌细胞系可组成性产生具有生物活性的LIF。本文讨论了癌细胞产生LIF可能的生物学意义。
