Law S K, Micklem K J, Shaw J M, Zhang X P, Dong Y, Willis A C, Mason D Y
Department of Biochemistry, University of Oxford, GB.
Eur J Immunol. 1993 Sep;23(9):2320-5. doi: 10.1002/eji.1830230940.
The antibodies Ki-M8, Ber-Mac3, GHI/61 and SM4 define a human macrophage-associated antigen with a relative molecular mass of 130,000 which we designate M130. The protein was purified by immunoaffinity chromatography and an N-terminal and three internal amino acid sequences were obtained. A cDNA fragment was initially obtained by polymerase chain reaction (PCR) using reverse-translated primers. Several variant cDNA clones, derived from alternative spliced messages, were obtained from a lipopolysaccharide-stimulated human monocyte library and were sequenced. The relative abundance of these variants was evaluated by a series of overlapping PCR reactions. The size of the most representative cDNA is 3.7 kb and closely agrees with the mRNA size of 3.8 kb determined by Northern blot analysis. The membrane protein encoded contains a leader peptide of 40 residues, a putative extracellular domain of 1003 residues, followed by a hydrophobic segment of 24 residues and a cytoplasmic domain of 49 residues. The extracellular domain was found to contain nine repeating elements, of about 110 residues, which are similar to those of the scavenger receptor superfamily.
抗体Ki-M8、Ber-Mac3、GHI/61和SM4鉴定出一种相对分子质量为130,000的人巨噬细胞相关抗原，我们将其命名为M130。该蛋白通过免疫亲和层析法纯化，并获得了N端和三个内部氨基酸序列。最初使用反向翻译引物通过聚合酶链反应（PCR）获得了一个cDNA片段。从脂多糖刺激的人单核细胞文库中获得了几个源自可变剪接信息的可变cDNA克隆，并进行了测序。通过一系列重叠PCR反应评估了这些变体的相对丰度。最具代表性的cDNA大小为3.7 kb，与通过Northern印迹分析确定的3.8 kb mRNA大小非常吻合。编码的膜蛋白包含一个40个残基的前导肽、一个1003个残基的假定细胞外结构域，接着是一个24个残基的疏水片段和一个49个残基的细胞质结构域。发现细胞外结构域包含九个约110个残基的重复元件，与清道夫受体超家族的元件相似。
