Vodkin M H, Gordon V R, McLaughlin G L
Department of Veterinary Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907.
Gene. 1993 Sep 6;131(1):141-4. doi: 10.1016/0378-1119(93)90683-t.
An EcoRI fragment from the mitochondrial DNA of Acanthamoeba polyphaga was cloned and partly sequenced, and the conceptual translation product of the open reading frame (partial sequence) was found to have similarities with rp114, a ribosomal protein. Phylogenetic analysis based on the amino acid (aa) sequences of this conserved protein resolved four branches that consisted of: (1) eubacteria and the chloroplasts of algae and higher plants, (2) ciliate mitochondria, (3) Acanthamoeba, and (4) archaebacteria and the nuclei of eukaryotes. The groupings based on the rp114 aa sequences were consistent with the phylogenies derived by rRNA analysis of these organisms.
从多噬棘阿米巴线粒体DNA中克隆出一个EcoRI片段并进行了部分测序，发现开放阅读框（部分序列）的概念性翻译产物与核糖体蛋白rp114有相似性。基于这种保守蛋白的氨基酸（aa）序列进行的系统发育分析解析出四个分支，它们分别由以下部分组成：（1）真细菌以及藻类和高等植物的叶绿体，（2）纤毛虫线粒体，（3）棘阿米巴，以及（4）古细菌和真核生物的细胞核。基于rp114氨基酸序列的分组与通过对这些生物进行rRNA分析得出的系统发育关系一致。
