一个核糖体蛋白基因簇编码在盘基网柄菌的线粒体DNA中：UGA终止密码子以及与卡氏棘阿米巴基因顺序的相似性。

A ribosomal protein gene cluster is encoded in the mitochondrial DNA of Dictyostelium discoideum: UGA termination codons and similarity of gene order to Acanthamoeba castellanii.

作者信息

Iwamoto M, Pi M, Kurihara M, Morio T, Tanaka Y

机构信息

Institute of Biological Sciences, University of Tsukuba, Japan.

出版信息

Curr Genet. 1998 Apr;33(4):304-10. doi: 10.1007/s002940050341.

DOI:10.1007/s002940050341

PMID:9560439

Abstract

We sequenced a region of about 14.5 kb downstream from the ribosomal protein L11 gene (rpl11) in the mitochondrial DNA (54+/-2 kb) of the cellular slime mold Dictyostelium discoideum. Sequence analysis revealed that eleven ribosomal protein genes and six open reading frames (ORFs) formed a cluster arranged in the order: rpl11-orf189-rps12-rps7-rpl2-rps19-+ ++orf425-orf1740-rpl16-rpl14-orf188- rps14-rps8-rpl6-rps13-orf127-orf796. This order was very similar to that of homologous genes in Acanthamoeba castellanii mitochondrial DNA. The N-terminal region of ORF425 and the C-terminal region of ORF1740 had partial similarities to the S3 ribosomal protein of other organisms. The termination codons of rpl16 and orf188 were UGA, which has not hitherto been found in genes encoded in D. discoideum mitochondrial DNA.

摘要

我们对细胞黏菌盘基网柄菌线粒体DNA（54±2 kb）中核糖体蛋白L11基因（rpl11）下游约14.5 kb的区域进行了测序。序列分析显示，11个核糖体蛋白基因和6个开放阅读框（ORF）形成了一个簇，排列顺序为：rpl11-orf189-rps12-rps7-rpl2-rps19-+++orf425-orf1740-rpl16-rpl14-orf188-rps14-rps8-rpl6-rps13-orf127-orf796。这个顺序与卡氏棘阿米巴线粒体DNA中同源基因的顺序非常相似。ORF425的N端区域和ORF1740的C端区域与其他生物的S3核糖体蛋白有部分相似性。rpl16和orf188的终止密码子是UGA，这在盘基网柄菌线粒体DNA编码的基因中尚未发现。