Okamoto Y, Ogawa K, Motohiro T, Nishi N, Muta E, Ota S
Department of Biochemistry, Daiichi College of Pharmaceutical Sciences, Fukuoka.
J Biochem. 1993 Jun;113(6):658-64. doi: 10.1093/oxfordjournals.jbchem.a124099.
Spotted mackerel protamine, scombrine, was isolated from the sperm of a spotted mackerel (Scomber australasicus) by extraction with sulfuric acid and fractionated into one major (scombrine II) and one minor (scombrine I) components by chromatography on CM-Sephadex C-25. Scombrine II gave a single band, whereas scombrine I gave three bands upon PAGE. Scombrine II (scombrine gamma) consists of 34 amino acid residues, and its sequence is: Pro-Arg-Arg-Arg-Arg-Arg-Ala-Ser-Arg-Pro-Val-Arg-Arg-Arg-Arg-Arg- Ala-Arg-Arg-Ser-Thr-Ala-Val-Arg-Arg-Arg-Arg-Arg-Val-Val-Arg-Arg-Arg-Arg. The ion spray mass spectrum shows that scombrine gamma has a molecular mass of 4,532.13 Da. The other minor component (scombrine I) is considered to be a mixture of degradation products of scombrine gamma based on the results of PAGE and ion spray mass spectrometry. Scombrine gamma has a similar sequence to sardaine Z2 from striped bonito except for two positions.
斑点鲭鱼精蛋白——鲭精蛋白，是通过用硫酸萃取从斑点鲭鱼（澳洲鲭）的精子中分离得到的，并通过在CM-葡聚糖凝胶C-25上进行色谱分离，分成一个主要成分（鲭精蛋白II）和一个次要成分（鲭精蛋白I）。在聚丙烯酰胺凝胶电泳（PAGE）中，鲭精蛋白II呈现一条带，而鲭精蛋白I呈现三条带。鲭精蛋白II（鲭精蛋白γ）由34个氨基酸残基组成，其序列为：脯氨酸-精氨酸-精氨酸-精氨酸-精氨酸-精氨酸-丙氨酸-丝氨酸-精氨酸-脯氨酸-缬氨酸-精氨酸-精氨酸-精氨酸-精氨酸-精氨酸-丙氨酸-精氨酸-精氨酸-丝氨酸-苏氨酸-丙氨酸-缬氨酸-精氨酸-精氨酸-精氨酸-精氨酸-精氨酸-缬氨酸-缬氨酸-精氨酸-精氨酸-精氨酸-精氨酸。离子喷雾质谱显示鲭精蛋白γ的分子量为4532.13道尔顿。基于PAGE和离子喷雾质谱的结果，另一个次要成分（鲭精蛋白I）被认为是鲭精蛋白γ的降解产物混合物。除了两个位置外，鲭精蛋白γ与条纹鲣的沙丁精蛋白Z2具有相似的序列。
