用于检测产肠毒素大肠杆菌定居因子抗原的传统培养基的评估
Evaluation of conventional media for detection of colonization factor antigens of enterotoxigenic Escherichia coli.
作者信息
Ghosh A R, Sen D, Sack D A, Hoque A T
机构信息
National Institute of Cholera and Enteric Diseases, Calcutta, India.
出版信息
J Clin Microbiol. 1993 Aug;31(8):2163-6. doi: 10.1128/jcm.31.8.2163-2166.1993.
Abstract
Strains of enterotoxigenic Escherichia coli producing either colonization factor antigen (CFA) I or II were tested for expression of CFA when grown on 16 different agar media by using agglutination and coagglutination with monoclonal antibodies, mannose-resistant hemagglutination, and a salt aggregation assay. CFA was detected from the CFA-positive strains when CFA agar was used, and it was also detected when other commercially available media were used, notably nutrient agar. CFA was not detected when other commercial media such as MacConkey agar were used. The use of nutrient agar with monoclonal antibody-based coagglutination reagents offers a potentially simple and rapid method for detecting E. coli which express CFA I or II.
摘要
采用单克隆抗体进行凝集和协同凝集、甘露糖抗性血凝以及盐凝集试验，检测了产生定居因子抗原（CFA）Ⅰ或Ⅱ的产肠毒素大肠杆菌菌株在16种不同琼脂培养基上生长时CFA的表达情况。当使用CFA琼脂时，可从CFA阳性菌株中检测到CFA；使用其他市售培养基（尤其是营养琼脂）时，也能检测到CFA。使用麦康凯琼脂等其他市售培养基时未检测到CFA。使用营养琼脂和基于单克隆抗体的协同凝集试剂，为检测表达CFAⅠ或Ⅱ的大肠杆菌提供了一种可能简单且快速的方法。
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