Levine R L
Department of Biology, McGill University, Montreal, Quebec, Canada.
J Comp Neurol. 1993 Jul 22;333(4):543-53. doi: 10.1002/cne.903330407.
During optic fiber regeneration in the goldfish, astrocytes in the visual system undergo a number of changes. These include hypertrophy of cell processes, increased reactivity with anti-intermediate filament antisera, and expression of cytoskeletal antigens not usually seen in these cells. In the present study, I have asked how much of this response might be due to interactions of glial cells with regenerating optic axons. Animals with and without a retina (regenerating and nonregenerating animals, respectively) had their optic nerve crushed and were then examined at various postoperative times with immunohistochemical methods. Three major differences between these two groups of animals were observed. First, in nonregenerating animals the crush lesion is not repopulated by immunoreactive glial cells while in regenerating animals it is. Second, the nature of the glial hypertrophy in the optic nerve is different in regenerating and nonregenerating animals. Finally, there is marked submeningeal swelling in regenerating nerves that is absent from nonregenerating nerves. Thus, these three aspects of the cellular response to optic nerve crush in the goldfish--wound healing, optic nerve gliosis, and non-neural cellular responses--appear to depend on interactions between regenerating optic axons and astrocytes or other non-neuronal cells of the visual paths for their expression.
在金鱼视神经再生过程中，视觉系统中的星形胶质细胞会发生一系列变化。这些变化包括细胞突起肥大、与抗中间丝抗血清的反应性增加，以及这些细胞中通常未见的细胞骨架抗原的表达。在本研究中，我探讨了这种反应在多大程度上可能是由于神经胶质细胞与再生视神经轴突的相互作用所致。分别对视神经被挤压的有视网膜和无视网膜的动物（分别为再生动物和非再生动物）进行观察，并在术后不同时间用免疫组织化学方法进行检测。观察到这两组动物之间存在三个主要差异。第一，在非再生动物中，挤压损伤部位没有免疫反应性神经胶质细胞重新填充，而在再生动物中则有。第二，再生动物和非再生动物视神经中神经胶质细胞肥大的性质不同。最后，再生神经中有明显的蛛网膜下肿胀，而非再生神经中则没有。因此，金鱼对视神经挤压的细胞反应的这三个方面——伤口愈合、视神经胶质增生和非神经细胞反应——其表现似乎依赖于再生视神经轴突与星形胶质细胞或视觉通路中其他非神经元细胞之间的相互作用。
