Favre N, Erb P
Institute for Medical Microbiology, University of Basel, Switzerland.
J Immunol Methods. 1993 Sep 15;164(2):213-20. doi: 10.1016/0022-1759(93)90314-w.
The isolation and characterization of a new and excellent indicator cell line for murine interleukin-4 (IL-4) bioassays, CTL44, is described. CTL44, a subline of CTL/L cells, is vigorously responsive to murine IL-4, but hyporesponsive to IL-2, requiring > 6 ng/ml (approximately 120 U/ml) of human IL-2 or > 80 U/ml of mouse IL-2 to induce IL-2 dependent proliferation. In CTL44 both IL-4 receptor mRNA accumulation and cell surface expression are detected, whereas IL-2 receptor expression appears to be absent. CTL44 cells maintained in IL-4 containing medium grow rapidly in a non-adherent fashion, and can be stored frozen in liquid nitrogen without loss of function.
本文描述了一种用于小鼠白细胞介素-4（IL-4）生物测定的新型优良指示细胞系CTL44的分离与特性鉴定。CTL44是CTL/L细胞的一个亚系，对小鼠IL-4反应强烈，但对IL-2反应较弱，诱导IL-2依赖性增殖需要>6 ng/ml（约120 U/ml）的人IL-2或>80 U/ml的小鼠IL-2。在CTL44中可检测到IL-4受体mRNA积累和细胞表面表达，而IL-2受体表达似乎不存在。在含IL-4的培养基中培养的CTL44细胞以非贴壁方式快速生长，并且可以液氮冻存而不丧失功能。
