Trautinger F, Trautinger I, Kindas-Mügge I, Metze D, Luger T A
Ludwig Boltzmann Institute-DVS, Department of Dermatology, University of Muenster, Germany.
J Invest Dermatol. 1993 Sep;101(3):334-8. doi: 10.1111/1523-1747.ep12365491.
Exposure of cells to elevated temperatures induces a physiologic response characterized by the synthesis of a specific set of proteins (heat shock or stress proteins, HSPs) mediating repair mechanisms and protection from cellular damage. In the present study upon immunohistochemistry using a specific monoclonal antibody, the constitutive and heat-induced expression of the 72-kD HSP (HSP72) in normal human skin and in human epidermal cell lines (KB, A431) was investigated. Normal (unstressed) epidermis and adnexal structures of normal human skin were found to constitutively express HSP72. In contrast, a substantial HSP72 expression could not be observed in the dermal cellular compartment. In vitro heat treatment of punch biopsies from normal skin (42 degrees C, 4 h) resulted in a further increase of epidermal HSP72 expression. In addition, dermal cells were found to be induced to express HSP72. To further evaluate the spontaneous HSP72 expression of epidermal cells two epidermoid carcinoma cell lines (A431, KB) were investigated. Upon immunohistochemistry and Western blot analysis a significant HSP72 expression could be detected in unstressed KB and A431 cells. In contrast, a human fibrosarcoma cell line (HT1080) was negative for HSP72 at 37 degrees C but upon heat treatment a strong induction was observed. Furthermore, Northern blot analysis using a cDNA probe specific for human HSP72 revealed a constitutive expression of HSP72 mRNA in both epidermal cell lines. These findings demonstrate a significant expression of the stress-inducible HSP72 in unstressed human skin as well as in epidermal cell lines, suggesting that HSP72 may inherently be involved in the protective function of normal human skin.
将细胞暴露于高温下会诱导一种生理反应，其特征是合成一组特定的蛋白质（热休克蛋白或应激蛋白，HSPs），这些蛋白质介导修复机制并保护细胞免受损伤。在本研究中，使用特异性单克隆抗体进行免疫组织化学，研究了72-kD热休克蛋白（HSP72）在正常人皮肤和人表皮细胞系（KB、A431）中的组成性和热诱导表达。发现正常人皮肤的正常（未应激）表皮和附属结构组成性表达HSP72。相比之下，在真皮细胞区室中未观察到大量的HSP72表达。对正常皮肤的打孔活检组织进行体外热处理（42℃，4小时）导致表皮HSP72表达进一步增加。此外，发现真皮细胞被诱导表达HSP72。为了进一步评估表皮细胞的自发HSP72表达，研究了两种表皮样癌细胞系（A431、KB）。通过免疫组织化学和蛋白质印迹分析，在未应激的KB和A431细胞中可检测到显著的HSP72表达。相比之下，人纤维肉瘤细胞系（HT1080）在37℃时HSP72呈阴性，但热处理后观察到强烈诱导。此外，使用针对人HSP72的cDNA探针进行Northern印迹分析，发现在两种表皮细胞系中均有HSP72 mRNA的组成性表达。这些发现表明，应激诱导的HSP72在未应激的人皮肤以及表皮细胞系中均有显著表达，提示HSP72可能固有地参与正常人皮肤的保护功能。
