Edwards M J, Marks R, Dykes P J, Merrett V R, Morgan H E, O'Donovan M R
Department of Medicine (Dermatology), University of Wales College of Medicine, Heath Park, Cardiff, U.K.
J Invest Dermatol. 1991 Mar;96(3):392-6. doi: 10.1111/1523-1747.ep12466250.
Heat shock induces in cells the synthesis of specific proteins called heat-shock proteins. We have compared the induction of these proteins in human keratinocytes, skin fibroblasts, and a human epithelial tumor cell line following exposure to weak and strong inducing agents (heat, cadmium sulphate, and sodium arsenite). The induction of heat shock proteins was measured in cells by one-dimensional gel electrophoresis of [35S] methionine-labeled proteins and by immunofluorescence using a specific HSP72 monoclonal antibody. Both HSP90 and HSP116 were constitutively expressed in these cell types. Exposure of these cells to weak inducing agents such as heat or cadmium sulphate resulted in the synthesis of HSP72 and HSP90, whereas HSP28 and HSP116 synthesis was detected in keratinocytes and fibroblasts following exposure to the strong inducing agent sodium arsenite. In addition, sodium arsenite induced the synthesis of HSP46 in human keratinocytes. Immunofluorescence demonstrated a rapid and reversible accumulation of the 72-kD heat shock protein within the nucleolus of heat-stressed human keratinocytes and fibroblasts.
热休克可诱导细胞合成一类特定的蛋白质，即热休克蛋白。我们比较了人类角质形成细胞、皮肤成纤维细胞以及一种人类上皮肿瘤细胞系在暴露于弱诱导剂和强诱导剂（热、硫酸镉和亚砷酸钠）后这些蛋白质的诱导情况。通过对[35S]甲硫氨酸标记的蛋白质进行一维凝胶电泳以及使用特异性HSP72单克隆抗体进行免疫荧光检测来测定细胞中热休克蛋白的诱导情况。HSP90和HSP116在这些细胞类型中均组成性表达。将这些细胞暴露于弱诱导剂如热或硫酸镉会导致HSP72和HSP90的合成，而在暴露于强诱导剂亚砷酸钠后，角质形成细胞和成纤维细胞中可检测到HSP28和HSP116的合成。此外，亚砷酸钠可诱导人类角质形成细胞中HSP46的合成。免疫荧光显示，热应激的人类角质形成细胞和成纤维细胞的核仁内72-kD热休克蛋白迅速且可逆地积累。
