Krivastava K K, Foster J W
Can J Microbiol. 1977 Feb;23(2):197-201. doi: 10.1139/m77-028.
An immunogenic fraction (IF) of Pasteurella multocida strain P-1059 was separated from culture filtrate by Sephadex gel filtration. Additional fractionation of IF with aqueous ether resulted in the glycoprotein-like preparation (GLP) while extraction with aqueous phenol provided the lipopolysaccharide-like preparation (LPP). The unextracted IF contained carbohydrate, protein, and lipid; the GLP contained carbohydrate and protein; and the LPP contained carbohydrate and lipid. The GLP was maximally protective for mice against homologous challenge, and was medially toxic in rabbit skin when compared to the other culture-filtrate preparations; the LPP was maximally toxic in rabbit skin, and was least protective for mice; and the unextracted IF was medially protective for mice, and was least toxic in rabbit skin.
多杀巴斯德氏菌P - 1059菌株的免疫原性组分（IF）通过葡聚糖凝胶过滤从培养滤液中分离出来。用乙醚水溶液对IF进行进一步分级分离得到类糖蛋白制剂（GLP），而用苯酚水溶液提取则得到类脂多糖制剂（LPP）。未提取的IF含有碳水化合物、蛋白质和脂质；GLP含有碳水化合物和蛋白质；LPP含有碳水化合物和脂质。GLP对小鼠抵抗同源攻击具有最大的保护作用，与其他培养滤液制剂相比，在兔皮中的毒性中等；LPP在兔皮中的毒性最大，对小鼠的保护作用最小；未提取的IF对小鼠具有中等保护作用，在兔皮中的毒性最小。
