Baba T
Infect Immun. 1977 Jan;15(1):1-6. doi: 10.1128/iai.15.1.1-6.1977.
The cells of P. multocida strain P-1059 were destroyed with the French pressure cell; the ribosomal fraction proven to be homogeneous by analytical ultracentrifugation was obtained from the product by centrifugal fractionation, zonal electrophoresis, and Sephadex G-200 gel filtration. The ribosomal fraction exhibited intense protective antigenicity in mice and chickens, but the lipopolysaccharide (endotoxin) and the other bacterial cell fraction obtained in this experiment did not. Sodium deoxycholate treatment of the ribosomal fraction resulted in only a 13% loss in immunological activity, and ribonuclease treatment caused a 60% loss of activity.
多杀性巴氏杆菌P-1059菌株的细胞用法国压榨器破碎；通过分析超速离心证明为均一的核糖体组分，是通过离心分级、区带电泳和葡聚糖凝胶G-200凝胶过滤从产物中获得的。该核糖体组分在小鼠和鸡中表现出强烈的保护性抗原性，但本实验中获得的脂多糖（内毒素）和其他细菌细胞组分则没有。用脱氧胆酸钠处理核糖体组分仅导致免疫活性损失13%，而用核糖核酸酶处理则导致活性损失60%。
