Negami A I, Sasaki H, Tominaga T
Department of Obstetrics and Gynecology, Fukui Medical School, Japan.
Am J Obstet Gynecol. 1993 Sep;169(3):739-43. doi: 10.1016/0002-9378(93)90655-3.
To demonstrate the direct effect of danazol on the endometrium in the presence of various types of extracellular matrix.
Human endometrial cells (epithelial and stromal cells) were cultured over various extracellular matrix in the absence or presence of danazol. The effects of danazol on the growth of endometrial cells and the specific glandular structure were studied.
Danazol (10(-7) mol/L) inhibited endometrial cell growth, and in the presence of Matrigel, the same danazol concentration demonstrated a stronger inhibitory effect on epithelial cell growth. Danazol inhibited the formation of the specific glandular structure. Its effect depended on the culture substrates used and was reversible. Danazol did not inhibit growth of cells cultured over type V collagen substrate, whereas growth of those grown over type IV collagen was greatly inhibited.
The results indicate that a distinctive extracellular matrix around ectopic endometriotic tissue modulates cell growth and differentiation. Endometrium associated with a normal extracellular matrix is apparently protected from therapeutic drugs, whereas endometriotic tissue growing over a deranged extracellular matrix would be sensitive to therapeutic drugs, such as danazol.
证明达那唑在存在各种类型细胞外基质的情况下对子宫内膜的直接作用。
在不存在或存在达那唑的情况下，将人子宫内膜细胞（上皮细胞和基质细胞）培养于各种细胞外基质上。研究了达那唑对子宫内膜细胞生长和特定腺结构的影响。
达那唑（10⁻⁷mol/L）抑制子宫内膜细胞生长，并且在基质胶存在的情况下，相同浓度的达那唑对上皮细胞生长表现出更强的抑制作用。达那唑抑制特定腺结构的形成。其作用取决于所用的培养底物，并且是可逆的。达那唑不抑制在V型胶原底物上培养的细胞生长，而在IV型胶原上生长的细胞生长则受到极大抑制。
结果表明，异位子宫内膜组织周围独特的细胞外基质调节细胞生长和分化。与正常细胞外基质相关的子宫内膜显然免受治疗药物的影响，而在紊乱的细胞外基质上生长的子宫内膜组织对达那唑等治疗药物敏感。
