Viganò P, Di Blasio A M, Busacca M, Vignali M
II Department of Obstetrics and Gynecology, Clinica 'La Mangiagalli', University of Milan, Italy.
Gynecol Endocrinol. 1994 Mar;8(1):13-9. doi: 10.3109/09513599409028452.
The influence of danazol on the expression of lymphocyte-mediated cytotoxicity toward the stromal component of the endometrium was examined. Stromal cells were isolated from endometrial biopsy specimens of nine women without endometrial pathology and 12 patients with endometriosis. A cytotoxicity assay by 51Cr release was performed to determine the effect of danazol on natural killer lymphocyte lytic activity. A significant suppression of spontaneous cytotoxicity against stromal cell targets of both study subjects was observed when effectors were pretreated for 48 h with danazol at a concentration of 5 x 10(-6) mol/l. The time-course of the danazol effect indicated that drug activity was detectable as early as 24 h after the addition of the drug and increase up to 72 h. These findings confirm that danazol may influence the immune system at many levels. In particular, they demonstrate that the drug is also able to inhibit the natural immunity toward the stromal component of the endometrium.
研究了达那唑对淋巴细胞介导的针对子宫内膜基质成分的细胞毒性表达的影响。从9名无子宫内膜病变的女性和12名子宫内膜异位症患者的子宫内膜活检标本中分离出基质细胞。通过51Cr释放进行细胞毒性测定，以确定达那唑对自然杀伤淋巴细胞裂解活性的影响。当效应细胞用浓度为5×10(-6)mol/L的达那唑预处理48小时时，观察到两个研究对象对基质细胞靶标的自发细胞毒性均有显著抑制。达那唑作用的时间进程表明，早在添加药物后24小时即可检测到药物活性，并持续增加至72小时。这些发现证实达那唑可能在多个层面影响免疫系统。特别是，它们表明该药物还能够抑制针对子宫内膜基质成分的天然免疫。
