O'Shaughnessy P J, Dudley K
Department of Veterinary Physiology, Veterinary School, University of Glasgow, UK.
J Mol Endocrinol. 1993 Jun;10(3):363-6. doi: 10.1677/jme.0.0100363.
The structure of RNA encoding the mouse testis FSH receptor was studied using reverse transcription and the polymerase chain reaction. Four major bands were observed by ethidium bromide staining and by hybridization to an FSH-receptor cDNA probe. The largest of these bands was the expected size (779 bp) while the other bands were spaced approximately 70 bp apart. Using alternative primers, each of the products was shown to contain exons 1, 9 and 10. Exons 2-8 in the FSH receptor gene are between 68 and 77 bp in size, suggesting that these multiple products arise by alternate splicing of the region encoding the extracellular domain of the receptor. A similar pattern of splicing was observed in cDNA from the testes of hypogonadal mice, showing that this alternative splicing pattern is not gonadotrophin-dependent.
利用逆转录和聚合酶链反应研究了编码小鼠睾丸促卵泡激素（FSH）受体的RNA结构。通过溴化乙锭染色和与FSH受体cDNA探针杂交观察到四条主要条带。其中最大的条带为预期大小（779 bp），而其他条带间隔约70 bp。使用替代引物，显示每个产物都包含外显子1、9和10。FSH受体基因中的外显子2 - 8大小在68至77 bp之间，这表明这些多种产物是由受体细胞外结构域编码区域的交替剪接产生的。在性腺功能减退小鼠睾丸的cDNA中观察到类似的剪接模式，表明这种交替剪接模式不依赖促性腺激素。
