一种用于DNA向下碱性Southern印迹的快速优化方案。
A rapid optimized protocol for downward alkaline Southern blotting of DNA.
作者信息
Koetsier P A, Schorr J, Doerfler W
机构信息
Institute for Genetics, University of Cologne, FRG.
出版信息
Biotechniques. 1993 Aug;15(2):260-2.
PMID:8373591
Abstract
A simple and efficient 2.5-h Southern blotting procedure is described that uses 0.4 M NaOH to transfer DNA in a downward direction. The resulting blots give signals that are both sharper and 30% stronger than those obtained by conventional upward-transferred blots.
摘要
本文描述了一种简单高效的2.5小时Southern印迹法，该方法使用0.4 M氢氧化钠向下转移DNA。所得印迹产生的信号比传统向上转移印迹获得的信号更清晰，强度高出30%。
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