用于小鼠κ可变区PCR扩增的扩展引物组。
An extended primer set for PCR amplification of murine kappa variable regions.
作者信息
Leung S O, Dion A S, Pellegrini M C, Goldenberg D M, Hansen H J
机构信息
Garden State Cancer Center, Center for Molecular Medicine and Immunology, Newark, NJ.
出版信息
Biotechniques. 1993 Aug;15(2):286-92.
PMID:8373597
Abstract
The design and successful usage of an extended primer set for the PCR amplification of murine variable kappa light chain sequences from mouse hybridomas are described. Since some of these primer pairs also amplify the endogenous SP2/0 aberrant light chain sequence, strategies to distinguish the irrelevant SP2/0 from the sequence of interest are also provided.
摘要
本文描述了用于从小鼠杂交瘤中PCR扩增小鼠可变κ轻链序列的扩展引物组的设计及成功应用。由于其中一些引物对也能扩增内源性SP2/0异常轻链序列，因此还提供了区分无关的SP2/0与目标序列的策略。
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