Adzhalov V A, Stepchenko A G, Deev S A, Polianovskiĭ O L
Mol Biol (Mosk). 1987 Jul-Aug;21(4):1137-41.
The genome of hybridoma PTF-02 has two genes for the kappa chains, and only one of these codes for the synthesis of the antibody light chains. The nucleotide sequences corresponding to the leader peptide and to the variable region of this gene were determined. An amino acid sequence corresponding to exons has been proposed on the basis of the nucleotide sequence. A nucleotide sequence adjacent to the gene at the 5'-end has also been determined, in particular, the precise localization of TATA- and CAT-boxes as well as those of the conservative deca (dc) and pentadeca (pd) sequences. The structure of the regulatory region in the gene is similar to that in the myeloma genomes. However, the 5'-region differs in its nucleotide composition and in the frequency of dc sequences from the DNA sequences adjacent to the 5'-end of eukaryotic genes which do not belong to the immunoglobulin family.
杂交瘤PTF - 02的基因组中有两个κ链基因，其中只有一个编码抗体轻链的合成。确定了与该基因的前导肽和可变区相对应的核苷酸序列。根据核苷酸序列推测出了与外显子相对应的氨基酸序列。还确定了该基因5'端相邻的核苷酸序列，特别是TATA盒和CAT盒的精确位置以及保守的十聚体（dc）和十五聚体（pd）序列的位置。该基因调控区的结构与骨髓瘤基因组中的相似。然而，其5'区域在核苷酸组成以及dc序列的频率上与不属于免疫球蛋白家族的真核基因5'端相邻的DNA序列不同。
