Hazan B, Bern O, Carmel M, Lejbkowicz F, Goldstein R S, Nir U
Department of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.
Cell Growth Differ. 1993 Jun;4(6):443-9.
ferT is a mouse testis-specific mRNA, shown previously to potentially encode a 51 kilodalton tyrosine kinase termed p51ferT. The accumulation of ferT RNA is restricted to primary spermatocytes that are at the prophase stage of the first spermatogenic meiotic division. By using antibodies raised against a synthetic peptide which was designed according to a putative p51ferT unique amino acid sequence, we have shown that testicular cells indeed contain a 51 kilodalton protein that is recognized by the anti-p51ferT antibodies. The protein was not detected in six nontesticular mouse tissues, nor was it detected, like the ferT RNA, in the testes of 14-day-old mice. These findings strongly suggest that the 51 kilodalton protein is p51ferT. Immunohistochemical staining localized p51ferT to meiotically dividing spermatocytes. Transfection experiments in CHO cells confirmed the nuclear localization of p51ferT in eukaryotic cells. p51ferT seems thus to be the first meiosis-specific nuclear tyrosine kinase described to date.
ferT是一种小鼠睾丸特异性mRNA，先前已表明它可能编码一种名为p51ferT的51千道尔顿酪氨酸激酶。ferT RNA的积累仅限于处于第一次生精减数分裂前期的初级精母细胞。通过使用针对根据假定的p51ferT独特氨基酸序列设计的合成肽产生的抗体，我们已经表明睾丸细胞确实含有一种51千道尔顿的蛋白质，该蛋白质可被抗p51ferT抗体识别。在六种非睾丸小鼠组织中未检测到该蛋白质，在14日龄小鼠的睾丸中也未检测到该蛋白质，就像ferT RNA一样。这些发现强烈表明51千道尔顿的蛋白质是p51ferT。免疫组织化学染色将p51ferT定位到进行减数分裂的精母细胞。在CHO细胞中的转染实验证实了p51ferT在真核细胞中的核定位。因此，p51ferT似乎是迄今为止描述的第一种减数分裂特异性核酪氨酸激酶。
