Mantovani A
Cancer Res. 1977 Mar;37(3):815-20.
In virto exposure of murine lymphoma cells to adriamycin and daunomycin (DM) for 24 hr resulted in comparable cytotoxicity as assessed by viable cell counts and inhibition of DNA synthesis. The spontaneous DNA synthesis of spleen lymphocytes in vitro was also equally affected by the two agents. However, when the in vitro effects of the two drugs on macrophage monolayers were investigated, DM was found to be at least four times more toxic than AM. In vivo-administered DM was more suppressive than AM of Corynebacterium parvum-induced spleen macrophage cytotoxicity. The fact that DM is less effective than AM as a cancer chemotherapeutic agent in vivo may actually be due to the greater destruction of macrophages by DM and a resultant reduction in the contribution of host immunity to the anti-tumor action.
将鼠淋巴瘤细胞在体外暴露于阿霉素和柔红霉素（DM）24小时，通过活细胞计数和DNA合成抑制评估，结果显示二者具有相当的细胞毒性。这两种药物对体外脾淋巴细胞的自发DNA合成也有同等影响。然而，当研究这两种药物对巨噬细胞单层的体外作用时，发现DM的毒性至少是阿霉素（AM）的四倍。在体内给予时，DM对短小棒状杆菌诱导的脾巨噬细胞细胞毒性的抑制作用比AM更强。DM作为一种癌症化疗药物在体内不如AM有效的事实，实际上可能是由于DM对巨噬细胞的破坏更大，导致宿主免疫对抗肿瘤作用的贡献降低。
