Hafner G, Schinzel H, Ehrenthal W, Wagner C, Konheiser U, Zotz R, Lotz J, Blank R, Weilemann L S, Prellwitz W
Institute of Clinical Chemistry and Laboratory Medicine, Johannes Gutenberg University, Mainz, Germany.
Ann Hematol. 1993 Sep;67(3):121-5. doi: 10.1007/BF01701734.
To evaluate the influence of different blood sampling techniques on test results of thrombin-antithrombin III complex (TAT) and prothrombin fragment 1 + 2 (F1 + 2) serial determinations were performed. In six groups of nonrandomized patients (ten patients each) the concentrations of the coagulation markers of blood samples from central catheters (internal jugular, caval, Shaldon, pulmonary artery) and peripheral cannulas (17G and 18G) were compared with those of blood samples obtained simultaneously from direct venipunctures of the contralateral arm. Medians and 25th-75th percentiles of TAT and F1 + 2 concentrations of plasmas obtained from central catheters were not different from those taken from venipunctures. When delta mean values (catheter - venipuncture) were calculated negative results were obtained, indicating lower concentrations measured from blood sampled through central catheters with the exception of blood that taken from Shaldon catheters. Only for TAT concentrations significantly were lower values measured in blood samples taken from internal jugular catheters when compared with blood samples obtained from direct venipunctures. Significantly higher TAT concentrations were determined in blood samples obtained from Shaldon catheters. For both coagulation markers correlations were found between concentrations in blood samples from central catheters and venipunctures. In blood samples taken from peripheral venous cannulas only F1 + 2 concentrations correlated with the concentrations found in samples from direct venipuncture. In contrast to F1 + 2, TAT concentrations measured from blood samples via peripheral cannulas were determined significantly higher than those taken from direct venipunctures.(ABSTRACT TRUNCATED AT 250 WORDS)
为评估不同采血技术对凝血酶 - 抗凝血酶III复合物（TAT）和凝血酶原片段1 + 2（F1 + 2）检测结果的影响，进行了系列测定。在六组非随机分组的患者（每组10例）中，比较了从中心静脉导管（颈内静脉、腔静脉、Shaldon导管、肺动脉导管）和外周套管针（17G和18G）采集的血样中凝血标志物的浓度与同时从对侧手臂直接静脉穿刺采集的血样的浓度。从中心静脉导管获得的血浆中TAT和F1 + 2浓度的中位数及第25至75百分位数与静脉穿刺采集的血浆无差异。计算差值均值（导管 - 静脉穿刺）时得到阴性结果，表明除从Shaldon导管采集的血液外，通过中心静脉导管采集的血液中测得的浓度较低。仅颈内静脉导管采集的血样中TAT浓度与直接静脉穿刺采集的血样相比明显较低。从Shaldon导管采集的血样中测定的TAT浓度明显较高。对于这两种凝血标志物，在中心静脉导管和静脉穿刺采集的血样浓度之间均发现了相关性。在外周静脉套管针采集的血样中，仅F1 + 2浓度与直接静脉穿刺采集的血样中的浓度相关。与F1 + 2相反，通过外周套管针采集的血样中测得的TAT浓度明显高于直接静脉穿刺采集的血样。（摘要截断于250字）
