Huang X, Laurent P A, Zalma R, Pezerat H
Laboratoire de Réactivité de Surface et Structure, Université Pierre et Marie Curie, URA 1106 CNRS, Paris, France.
Chem Res Toxicol. 1993 Jul-Aug;6(4):452-8. doi: 10.1021/tx00034a011.
Increasing evidence demonstrates that emphysema in coal workers may be related to their exposure to coal dusts. The hypothesis that emphysema could be related to the production of reactive oxygen species (ROS) generated by inhaled coal dusts was examined in the present study. Using ESR, we investigated whether the interaction of different coals with dissolved oxygen in aqueous medium could generate ROS. Indeed, we found that one of the five examined French coal samples, Vouters coal, was effective in oxidizing formate anions or ethanol by a radical pathway. Inactivation of alpha 1-antitrypsin (alpha 1-AT) in vitro was then examined for all five coal filtrates. The Vouters coal filtrate, which exhibits oxidative activity, can also inactivate alpha 1-AT. When this coal filtrate was crystallized and redissolved, its oxidative activity was found to be conserved. By use of various analytical techniques, the active component of this coal filtrate was identified to be primarily ferrous sulfate. We confirmed that pure ferrous sulfate can effectively reduce oxygen to produce ROS in aqueous medium in vitro and can also inactivate alpha 1-AT. In this report, the nature of the coal-generated oxidative species, the origin of ferrous sulfate, and the stability of ferrous sulfate in the different coal samples are discussed. These results offer evidence that some inhaled coal dusts are capable of producing ROS, which may play an important role in the development of coal workers' emphysema.
越来越多的证据表明，煤矿工人的肺气肿可能与其接触煤尘有关。本研究检验了肺气肿可能与吸入煤尘产生的活性氧（ROS）有关这一假说。我们使用电子自旋共振（ESR）研究了不同煤炭与水介质中溶解氧的相互作用是否能产生活性氧。事实上，我们发现所检测的五个法国煤样之一——武特斯煤，能通过自由基途径有效氧化甲酸根阴离子或乙醇。随后我们对所有五个煤滤液进行了体外α1-抗胰蛋白酶（α1-AT）失活检测。具有氧化活性的武特斯煤滤液也能使α1-AT失活。当该煤滤液结晶并重新溶解后，其氧化活性得以保留。通过使用各种分析技术，确定该煤滤液的活性成分主要是硫酸亚铁。我们证实，纯硫酸亚铁在体外水介质中能有效还原氧气以产生活性氧，并且也能使α1-AT失活。在本报告中，我们讨论了煤产生的氧化物质的性质、硫酸亚铁的来源以及硫酸亚铁在不同煤样中的稳定性。这些结果提供了证据，表明一些吸入的煤尘能够产生活性氧，这可能在煤矿工人肺气肿的发展中起重要作用。
