Hixon W G, Searcy D G
Biology Department, University of Massachusetts, Amherst.
Biosystems. 1993;29(2-3):151-60. doi: 10.1016/0303-2647(93)90091-p.
Thermoplasma acidophilum has no cell wall, and so its irregular shape implies the presence of a cytoskeleton. When soluble extracts of T. acidophilum were incubated in vitro they increased in viscosity, suggestive of a polymerizable component. Optimal conditions for the viscosity increase coincided with physiological ionic concentrations. Electron micrographs of negatively stained extracts showed a meshlike lattice of elements 10 nm in diameter similar to nuclear lamins. However, immunologically there was no cross-reaction with lamins nor with the other eukaryotic cytoskeletal proteins tested: tubulin, calmodulin, giardin, actin or myosin.
嗜热栖热菌没有细胞壁，因此其不规则形状意味着存在细胞骨架。当嗜热栖热菌的可溶性提取物在体外孵育时，它们的粘度增加，这表明存在可聚合成分。粘度增加的最佳条件与生理离子浓度一致。对提取物进行负染色后的电子显微镜照片显示出直径为10纳米的元素组成的网状晶格，类似于核纤层蛋白。然而，在免疫方面，它与核纤层蛋白以及所测试的其他真核细胞骨架蛋白（微管蛋白、钙调蛋白、贾第虫蛋白、肌动蛋白或肌球蛋白）均无交叉反应。
