Chetverin A B, Kramer F R
Public Health Research Institute, New York, NY 10016.
Biosystems. 1993;30(1-3):215-31. doi: 10.1016/0303-2647(93)90072-k.
In sequencing-by-hybridization methods, the nucleotide sequence of a nucleic acid is reconstructed by overlapping oligonucleotides capable of hybridizing with the nucleic acid. In their present form, the methods are hardly suitable for sequencing of long nucleic acid molecules because of the occurrence of non-unique overlaps between the oligonucleotides, and similarly to the conventional sequencing methods, it is necessary to obtain an individual molecule. In the method described here, most ambiguities in reconstruction of a sequence from the constituent oligonucleotides are eliminated by preparing on oligonucleotide arrays and separate surveying of the nucleic acid nested partials. This enables longer nucleic acids to be sequenced, and results in a high redundancy of the input data allowing most hybridization errors to be eliminated by algorithmic means. Furthermore, large pools of nucleic acid strands can be sequenced directly, without isolating individual strands.
在杂交测序方法中，核酸的核苷酸序列是通过能够与该核酸杂交的重叠寡核苷酸来重建的。以其目前的形式，由于寡核苷酸之间存在非唯一重叠，这些方法几乎不适用于长核酸分子的测序，并且与传统测序方法类似，有必要获得单个分子。在此处描述的方法中，通过在寡核苷酸阵列上制备并单独检测核酸嵌套片段，消除了从组成寡核苷酸重建序列时的大多数模糊性。这使得更长的核酸能够被测序，并导致输入数据的高冗余性，从而允许通过算法手段消除大多数杂交错误。此外，可以直接对大量核酸链池进行测序，而无需分离单链。
