Haddad A, Nagai M E
Cell Tissue Res. 1977 Feb 15;177(3):347-69. doi: 10.1007/BF00220310.
L-fucose-3H was injected intravenously into mice which were killed at several time intervals after injection and semi-thin sections of their ovaries were processed for radioautography and analysed quantitatively; At the same time the specific activity of serum glycoproteins was determined. Glycoprotein biosynthesis was demonstrated in the oocytes, granulosa and stromal cells. The silver grain density of the follicular fluid in large follicles reached a peak at 4 h, remained high at 8 h after injection and decreased steadily at the subsequent intervals. It was demonstrated that the labeling pattern of the follicular fluid depends on the secretory activity of the granulosa cells and also on the specific activity of serum glycoproteins. The collapsed zonae pellucidae which represent the highest degree of follicular atresia are able to take up glycoprotein macromolecules. Based on this finding and also on the labeling pattern of the large follicles it was shown that there is very little synthesis of specific glycoproteins for the zona pellucida in large follicles. A more specific labeling of the zona pellucida occurred in the medium follicles. Following the growth of these follicles having a previously labeled zona pellucida, it was demonstrated that this extracellular structure is secreted by the oocyte.
将3H标记的L-岩藻糖静脉注射到小鼠体内，在注射后的几个时间间隔处死小鼠，对其卵巢进行半薄切片处理，用于放射自显影并进行定量分析；同时测定血清糖蛋白的比活性。在卵母细胞、颗粒细胞和基质细胞中证实了糖蛋白的生物合成。大卵泡中卵泡液的银粒密度在注射后4小时达到峰值，8小时时仍保持较高水平，随后各时间间隔稳步下降。结果表明，卵泡液的标记模式取决于颗粒细胞的分泌活性以及血清糖蛋白的比活性。代表卵泡闭锁最高程度的塌陷透明带能够摄取糖蛋白大分子。基于这一发现以及大卵泡的标记模式，结果显示大卵泡中透明带特异性糖蛋白的合成很少。中等大小卵泡的透明带出现了更特异性的标记。在这些先前标记过透明带的卵泡生长后，结果表明这种细胞外结构是由卵母细胞分泌的。
