Imerman P M
Iowa State University, Veterinary Diagnostic Laboratory, Ames 50010.
J AOAC Int. 1993 Jul-Aug;76(4):899-901.
A collaborative study of an indirect method for determination of cholinesterase activity was conducted with cattle blood. Ten collaborators analyzed 8 samples of varying degrees of cholinesterase inhibition. The sample is diluted with a buffer, and the pH change that results from the hydrolysis of acetylcholine is monitored. Repeatability relative standard deviations (RSDr) ranged from 6.6 to 8.6%, and reproducibility relative standard deviations (RSDR) ranged from 10.7 to 17.3% for mean activities of 0.60-0.40 delta pH/h. All samples that were completely inhibited were detected. The method was adopted first action by AOAC International for determination of cholinesterase activity in whole bovine blood.
用牛血对一种测定胆碱酯酶活性的间接方法进行了合作研究。十名合作者分析了8个胆碱酯酶抑制程度不同的样本。样本用缓冲液稀释，监测乙酰胆碱水解产生的pH变化。对于平均活性为0.60 - 0.40 ΔpH/h，重复性相对标准偏差（RSDr）范围为6.6%至8.6%，再现性相对标准偏差（RSDR）范围为10.7%至17.3%。所有完全抑制的样本都被检测到。该方法被美国官方分析化学师协会国际组织首次采用，用于测定全牛血中的胆碱酯酶活性。
