用[35S]-TBPS标记不同物种大脑中神经甾体结合位点的放射自显影研究。

Autoradiographic study of neurosteroid binding sites labelled with [35S]-TBPS in brain of different species.

作者信息

Vincens M, Dartois E, Popelier M, Marquette C, Moyse E, Fillion G, Haour F

机构信息

Pharmacologie Endocrinienne, Hopital Laribosiere, Paris, France.

出版信息

Brain Res Bull. 1993;32(3):251-6. doi: 10.1016/0361-9230(93)90184-d.

DOI:10.1016/0361-9230(93)90184-d

PMID:8374804

Abstract

Distribution of [35S]-TBPS binding sites was studied in various structures of brain in mouse and guinea pig and in cortex of monkey and in hippocampus of postmortem human brain. As it is observed for rat brain, high densities of [35S]-TBPS binding sites were found in layer IV of cortex in the four species, and in thalamus of mouse and guinea pig. Intermediate densities of binding sites were observed in superficial and deep layers of cortex in those four species and in hippocampus of mouse, guinea pig, and human. In all brain structures studied, 5 alpha 3 alpha P and picrotoxin produced a dose-dependent inhibition of [35S]-TBPS binding. No significant interregion or interspecies differences could not be detected for IC50 values of 5 alpha 3 alpha P or picrotoxin to inhibit [35S]-TBPS from its binding sites. In all regions studied, IC50 values were close to 1.5 x 10(-6) M for 5 alpha 3 alpha P and 2.3 x 10(-7) M for picrotoxin.

摘要

在小鼠、豚鼠的脑不同结构中，以及猴的皮质和人类尸检脑的海马体中研究了[35S] -TBPS结合位点的分布。正如在大鼠脑中观察到的那样，在这四个物种的皮质IV层以及小鼠和豚鼠的丘脑中发现了高密度的[35S] -TBPS结合位点。在这四个物种的皮质浅层和深层以及小鼠、豚鼠和人类的海马体中观察到中等密度的结合位点。在所有研究的脑结构中，5α3αP和印防己毒素对[35S] -TBPS结合产生剂量依赖性抑制。对于5α3αP或印防己毒素抑制[35S] -TBPS从其结合位点的IC50值，未检测到显著的区域间或物种间差异。在所有研究区域中，5α3αP的IC50值接近1.5×10(-6) M，印防己毒素的IC50值接近2.3×10(-7) M。