Jalauddin M, Campbell J B, Sanhueza J, Sesler A
Clin Biochem. 1977 Feb;10(1):38-43. doi: 10.1016/s0009-9120(77)90580-x.
Our experience with the Bio-Rad "Quanta-Count" folate radioassay kit has revealed very good in-run precision and good day-to-day reproducibility in the assay of both serum and red-cell folate levels. Ascorbic acid is not required as folate preservative if blood samples are frozen within hours after collection. For the determination of red-cell folates, our data clearly show the need for maintenance of a certain level (6-8 gm%) of protein in the assay system. Protein (albumin or folate-free human serum) must be added to the red-cell lysate to compensate for the serum loss resulting from the high dilution factor necessary. In the absence of this added protein, red-cell values are markedly lower. A good correlation exists between red-cell folate values obtained from the assay of washed red cells and from the assay of whole blood with corrections for serum folate levels.
我们使用伯乐“Quanta-Count”叶酸放射测定试剂盒的经验表明，在血清和红细胞叶酸水平测定中，该试剂盒具有非常好的批内精密度和良好的日间重复性。如果血液样本在采集后数小时内冷冻，则无需使用抗坏血酸作为叶酸防腐剂。对于红细胞叶酸的测定，我们的数据清楚地表明，在测定系统中需要维持一定水平（6-8克%）的蛋白质。必须向红细胞裂解液中添加蛋白质（白蛋白或无叶酸人血清），以补偿由于所需的高稀释倍数导致的血清损失。如果没有添加这种蛋白质，红细胞值会明显降低。通过洗涤红细胞测定获得的红细胞叶酸值与校正血清叶酸水平后全血测定获得的红细胞叶酸值之间存在良好的相关性。
