Mortensen E
Clin Chem. 1976 Jul;22(7):982-92.
Determination of the concentration of erythrocyte folate by means of competitive protein binding assay critically depends on the extraction procedure applied. Results will be influenced by variable factors such as the in vitro age of the blood samples, the degree of hemolysis, the presence of ascorbic acid, and the pH during extraction and elimination of proteins. The radioassay is strongly influenced by the pH of the final reaction mixture, the method used to separate free and protein-bound molecules, and the molecular configuration of the folates present. Based on experimental results presented, I describe a method for the determination of erythrocyte folate.
通过竞争性蛋白结合分析法测定红细胞叶酸浓度严重依赖于所采用的提取程序。结果会受到多种可变因素的影响，如血样的体外保存时间、溶血程度、抗坏血酸的存在以及提取和去除蛋白质过程中的pH值。放射免疫分析法会受到最终反应混合物的pH值、分离游离和蛋白质结合分子的方法以及所存在叶酸的分子构型的强烈影响。基于所呈现的实验结果，我描述了一种测定红细胞叶酸的方法。
