Trivin F, Odièvre M, Lemonnier A
Clin Chem. 1977 Mar;23(3):541-5.
Knowledge of the reserve bilirubin-binding capacity is useful in management of hyperbilirubinemia in the newborn. We describe thin-layer gel chromatography that permits measurement of this variable, with a 250-mul sample of serum, within 50 min. Single samples from four different infants can be analyzed on the same plate. The capacity of 40 sera from jaundiced and of 19 sera from nonjaundiced infants was measured. Results by thin-layer Sephadex gel chromatography and column Sephadex chromatography correlated well. Dithiothreitol, added to the standard bilirubin solution, protected bilirubin from oxidation for at least six days, which obviated the time consuming preparation of standard solution before such determination.
了解储备胆红素结合能力对新生儿高胆红素血症的管理很有用。我们描述了一种薄层凝胶色谱法，该方法可在50分钟内用250微升血清样本测量此变量。来自四个不同婴儿的单个样本可在同一平板上进行分析。我们测量了40份黄疸婴儿血清和19份非黄疸婴儿血清的结合能力。薄层葡聚糖凝胶色谱法和柱葡聚糖凝胶色谱法的结果相关性良好。添加到标准胆红素溶液中的二硫苏糖醇可保护胆红素至少六天不被氧化，这避免了在此类测定前耗时的标准溶液制备过程。
