猪鞭虫：来自成年寄生虫培养液的一种锌金属蛋白酶。
Trichuris suis: a zinc metalloprotease from culture fluids of adult parasites.
作者信息
Hill D E, Gamble H R, Rhoads M L, Fetterer R H, Urban J F
机构信息
United States Department of Agriculture, Beltsville Agricultural Research Center-East, Maryland 20705.
出版信息
Exp Parasitol. 1993 Sep;77(2):170-8. doi: 10.1006/expr.1993.1074.
PMID:8375486
Abstract
A zinc metalloendoprotease has been isolated from in vitro culture fluids of Trichuris suis adults. The protease was purified from total culture fluids by passage through a cation exchange high-pressure liquid chromatography column. The 45-kDa protease has a pH optimum of 7.0 and an isoelectric point of 8.0 and was localized to the stichosome of the parasites using immunohistochemistry techniques.
摘要
已从猪鞭虫成虫的体外培养液中分离出一种锌金属内蛋白酶。该蛋白酶通过阳离子交换高压液相色谱柱从总培养液中纯化得到。这种45 kDa的蛋白酶最适pH值为7.0，等电点为8.0，利用免疫组织化学技术定位到寄生虫的杆状体中。
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