Martin H, Patel Y, Jones D, Howell S, Robinson K, Aitken A
Laboratory of Protein Structure, National Institute for Medical Research, Mill Hill, London, UK.
FEBS Lett. 1993 Oct 4;331(3):296-303. doi: 10.1016/0014-5793(93)80356-y.
14-3-3 proteins are apparently ubiquitous eukaryotic proteins that comprise a large number of isoforms. They have been implicated in the regulation of a wide range of biological processes [reviewed in Aitken et al. (1992) Trends Biochem. Sci. 17, 498-501]. We have raised specific antibodies against each mammalian brain isoform of 14-3-3 employing peptides synthesised from the amino-terminal regions. The peptides were, like the proteins from mammalian brain, N-acetylated. The antiserum specific for the epsilon isoform did not recognise the recombinant form of this protein (lacking the N-acetyl co-translational modification) expressed in E. coli until it was chemically acetylated.
14-3-3蛋白显然是普遍存在于真核生物中的蛋白质，由大量的亚型组成。它们参与了广泛的生物过程的调控[见Aitken等人（1992年）《生物化学趋势》17卷，498 - 501页的综述]。我们利用从氨基末端区域合成的肽段，制备了针对14-3-3蛋白每种哺乳动物脑亚型的特异性抗体。这些肽段与哺乳动物脑中的蛋白质一样，都进行了N-乙酰化修饰。对ε亚型具有特异性的抗血清，在大肠杆菌中表达的该蛋白重组形式（缺乏N-乙酰共翻译修饰）未进行化学乙酰化处理之前，无法识别它。
