噬菌体φX174温度敏感型外部支架蛋白的第二位点抑制因子
Second-site suppressors of a cold-sensitive external scaffolding protein of bacteriophage phi X174.
作者信息
Fane B A, Shien S, Hayashi M
机构信息
Department of Biological Sciences, University of Arkansas, Fayetteville 72701.
出版信息
Genetics. 1993 Aug;134(4):1003-11. doi: 10.1093/genetics/134.4.1003.
Abstract
This report describes the isolation and characterization of second-site suppressors of a cold-sensitive (cs) external scaffolding protein, gpD, of bacteriophage phi X174. Seven genetically distinct suppressors were isolated. Six of them are located in gene F which encodes the major coat protein of the virus. The seventh is located in gene J which encodes the DNA-binding protein. A subset of the suppressors are trans-acting. These second-site suppressors do not exhibit allele specificity; they are able to suppress defects associated with a csD protein for which they were not selected. The initial characterization of the second-site suppressors and their locations within the major coat protein suggest that the mechanism of suppression may involve both structural and stoichiometric phenomena.
摘要
本报告描述了噬菌体φX174的冷敏感（cs）外部支架蛋白gpD的第二位点抑制子的分离和特性鉴定。分离出了七个遗传上不同的抑制子。其中六个位于编码病毒主要衣壳蛋白的基因F中。第七个位于编码DNA结合蛋白的基因J中。一部分抑制子具有反式作用。这些第二位点抑制子不表现出等位基因特异性；它们能够抑制与它们未被选择针对的csD蛋白相关的缺陷。第二位点抑制子的初步特性鉴定及其在主要衣壳蛋白中的位置表明，抑制机制可能涉及结构和化学计量现象。
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