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大肠杆菌OmpT蛋白酶对噬菌体phi X174原头部辅助蛋白gpB的蛋白水解作用对噬菌体在体内的成熟并非必不可少。

Proteolysis of bacteriophage phi X174 prohead accessory protein gpB by Escherichia coli OmpT protease is not essential for phage maturation in vivo.

作者信息

Dalphin M E, Fane B A, Skidmore M O, Hayashi M

机构信息

Department of Biology, University of California, San Diego, California 92093-0322.

出版信息

J Bacteriol. 1992 Apr;174(7):2404-6. doi: 10.1128/jb.174.7.2404-2406.1992.

DOI:10.1128/jb.174.7.2404-2406.1992
PMID:1532389
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC205867/
Abstract

To examine whether cleavage of the phi X174 prohead accessory protein, gpB, by the OmpT protease is required for phage development in vivo, a phage mutant lacking the OmpT cleavage site and an Escherichia coli C delta ompT strain were constructed. The results of burst size experiments suggest that neither the cleavage site nor the OmpT protein is required for phi X174 development.

摘要

为了检测OmpT蛋白酶对噬菌体φX174前头部辅助蛋白gpB的切割是否是噬菌体在体内发育所必需的，构建了一个缺乏OmpT切割位点的噬菌体突变体和一株大肠杆菌C ΔompT菌株。爆发量实验结果表明，切割位点和OmpT蛋白对于噬菌体φX174的发育都不是必需的。

相似文献

1
Proteolysis of bacteriophage phi X174 prohead accessory protein gpB by Escherichia coli OmpT protease is not essential for phage maturation in vivo.大肠杆菌OmpT蛋白酶对噬菌体phi X174原头部辅助蛋白gpB的蛋白水解作用对噬菌体在体内的成熟并非必不可少。
J Bacteriol. 1992 Apr;174(7):2404-6. doi: 10.1128/jb.174.7.2404-2406.1992.
2
Proteolysis of bacteriophage phi X174 prohead protein gpB by a protease located in the Escherichia coli outer membrane.位于大肠杆菌外膜的一种蛋白酶对噬菌体phi X174前头部蛋白gpB的蛋白水解作用。
J Bacteriol. 1988 Dec;170(12):5564-71. doi: 10.1128/jb.170.12.5564-5571.1988.
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Morphogenesis of phi X174: in vitro synthesis of infectious phage from purified viral components.噬菌体φX174的形态发生：从纯化的病毒成分体外合成感染性噬菌体。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7285-9. doi: 10.1073/pnas.78.12.7285.
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Isolation and identification of bacteriophage phi X174 prohead.噬菌体φX174原头部的分离与鉴定
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Mutational analysis of the bacteriophage phi X174 replication origin.噬菌体φX174复制起点的突变分析
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Functional relationship between the J proteins of bacteriophages phi X174 and G4 during phage morphogenesis.噬菌体φX174和G4的J蛋白在噬菌体形态发生过程中的功能关系。
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Alteration of the ATG start codon of the A protein of bacteriophage phi X174 into an ATT codon yields a viable phage indicating that A protein is not essential for phi X174 reproduction.将噬菌体φX174的A蛋白的ATG起始密码子改变为ATT密码子可产生一种有活力的噬菌体，这表明A蛋白对于φX174的繁殖并非必不可少。
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Mechanism of replication of bacteriophage phi X174. XXII. Site-specific mutagenesis of the A* gene reveals that A* protein is not essential for phi X174 DNA replication.噬菌体φX174的复制机制。XXII. A*基因的位点特异性诱变表明A*蛋白对φX174 DNA复制并非必需。
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In vitro packaging of plasmid DNAs into phi X174 bacteriophage capsid.
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引用本文的文献

1
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本文引用的文献

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Genetic Map of Bacteriophage phiX174.噬菌体φX174的遗传图谱
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P22 morphogenesis. I: Catalytic scaffolding protein in capsid assembly.P22形态发生。I：衣壳组装中的催化支架蛋白。
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Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and OmpT.对成对碱性残基具有特异性的大肠杆菌蛋白酶VII的纯化、特性鉴定及一级结构：蛋白酶VII与OmpT的同一性
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Proteolysis of bacteriophage phi X174 prohead protein gpB by a protease located in the Escherichia coli outer membrane.位于大肠杆菌外膜的一种蛋白酶对噬菌体phi X174前头部蛋白gpB的蛋白水解作用。
J Bacteriol. 1988 Dec;170(12):5564-71. doi: 10.1128/jb.170.12.5564-5571.1988.
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A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli.一组含有用于大肠杆菌基因图谱分析的遗传连锁交替抗生素抗性元件的菌株。
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Preliminary investigation of the phage phi X174 crystal structure.噬菌体φX174晶体结构的初步研究。
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