大肠杆菌OmpT蛋白酶对噬菌体phi X174原头部辅助蛋白gpB的蛋白水解作用对噬菌体在体内的成熟并非必不可少。
Proteolysis of bacteriophage phi X174 prohead accessory protein gpB by Escherichia coli OmpT protease is not essential for phage maturation in vivo.
作者信息
Dalphin M E, Fane B A, Skidmore M O, Hayashi M
机构信息
Department of Biology, University of California, San Diego, California 92093-0322.
出版信息
J Bacteriol. 1992 Apr;174(7):2404-6. doi: 10.1128/jb.174.7.2404-2406.1992.
Abstract
To examine whether cleavage of the phi X174 prohead accessory protein, gpB, by the OmpT protease is required for phage development in vivo, a phage mutant lacking the OmpT cleavage site and an Escherichia coli C delta ompT strain were constructed. The results of burst size experiments suggest that neither the cleavage site nor the OmpT protein is required for phi X174 development.
摘要
为了检测OmpT蛋白酶对噬菌体φX174前头部辅助蛋白gpB的切割是否是噬菌体在体内发育所必需的，构建了一个缺乏OmpT切割位点的噬菌体突变体和一株大肠杆菌C ΔompT菌株。爆发量实验结果表明，切割位点和OmpT蛋白对于噬菌体φX174的发育都不是必需的。
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