Langdon S P, Ritchie A, Young K, Crew A J, Sweeting V, Bramley T, Hillier S, Hawkins R A, Tesdale A L, Smyth J F
ICRF Medical Oncology Unit, Western General Hospital, Edinburgh, UK.
Int J Cancer. 1993 Sep 30;55(3):459-64. doi: 10.1002/ijc.2910550323.
A human ovarian adenocarcinoma cell line (PE04) has been established as a xenograft in nude mice. In vitro, this cell line is estrogen receptor (ER)-positive and its growth is stimulated by 17 beta-estradiol at concentrations between 10(-12) and 10(-6) M. When xenografted, PE04 cells remain ER-positive and also possess progesterone receptors (PR); treatment with 17 beta-estradiol reduces the concentration of ER and increases levels of PR. Growth of the xenograft is reduced in ovariectomized animals while implantation of estrogen pellets also results in growth inhibition. Similar treatment with estrogen does not inhibit the ER-negative HOX 60 ovarian xenograft, and stimulates growth of the ER-positive ZR-75-I breast carcinoma xenograft. Serum measurements of 17 beta-estradiol confirm that ovariectomy reduces the level of 17 beta-estradiol while implantation of estrogen pellets results in raised levels of the hormone. Tamoxifen inhibits growth of the PE04 xenograft but not that of the HOX 60 xenograft, consistent with ER status. These results indicate that ER-positive PE04 ovarian cancer cells are sensitive to 17 beta-estradiol in vivo but that the response may be of a different type from the in vitro response. This lends further support to the concept that ovarian cancer may be hormone-sensitive and potentially responsive to endocrine therapy.
一种人卵巢腺癌细胞系（PE04）已被建立为裸鼠异种移植模型。在体外，该细胞系雌激素受体（ER）呈阳性，其生长在10^(-12)至10^(-6) M浓度的17β-雌二醇刺激下加快。异种移植时，PE04细胞仍为ER阳性且也具有孕激素受体（PR）；用17β-雌二醇处理会降低ER浓度并增加PR水平。去卵巢动物体内异种移植瘤的生长减缓，而植入雌激素丸剂也会导致生长抑制。用雌激素进行类似处理不会抑制ER阴性的HOX 60卵巢异种移植瘤，反而会刺激ER阳性的ZR-75-I乳腺癌异种移植瘤的生长。血清17β-雌二醇检测证实，去卵巢会降低17β-雌二醇水平，而植入雌激素丸剂会使该激素水平升高。他莫昔芬抑制PE04异种移植瘤的生长，但不抑制HOX 60异种移植瘤的生长，这与ER状态一致。这些结果表明，ER阳性的PE04卵巢癌细胞在体内对17β-雌二醇敏感，但这种反应可能与体外反应类型不同。这进一步支持了卵巢癌可能对激素敏感且可能对内分泌治疗有反应的概念。
