Shi W, Lentz M J, Adler J
Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.
J Bacteriol. 1993 Sep;175(18):5785-90. doi: 10.1128/jb.175.18.5785-5790.1993.
The behavioral response of Escherichia coli to electric shock in 10(-2) M potassium phosphate plus 10(-4) M potassium EDTA was studied. When presented with a 150-V/cm electric shock that lasted 250 ms, the bacteria at first exclusively ran, then exclusively tumbled, and finally returned to their original running and tumbling. This response is due to increased temperature caused by the electric shock, i.e., to thermotaxis, and it is mediated by the chemotaxis machinery. A more severe electric shock, 150 V/cm for 550 ms, caused cells to tumble immediately, and then they went back to their original running and tumbling. The mechanism of that response is unknown since, unlike known thermotaxis, it does not require the chemotaxis machinery.
研究了大肠杆菌在10⁻² M磷酸钾加10⁻⁴ M乙二胺四乙酸钾中的电击行为反应。当受到持续250毫秒的150伏/厘米电击时,细菌起初只游动,然后只翻滚,最后恢复到原来的游动和翻滚状态。这种反应是由电击引起的温度升高导致的,即热趋性,并且由趋化机制介导。更强烈的电击,150伏/厘米持续550毫秒,会使细胞立即翻滚,然后它们又恢复到原来的游动和翻滚状态。这种反应的机制尚不清楚,因为与已知的热趋性不同,它不需要趋化机制。