胶质瘤囊液中正常脑反应性抗体的特征分析。

Characterization of normal brain-reactive antibodies in glioma cyst fluids.

作者信息

Brunet J M, Berger F, Gustin T, Lainé M, Benabid A L

机构信息

Laboratoire de Neurobiophysique, INSERM U318, Grenoble, France.

出版信息

J Neuroimmunol. 1993 Aug;47(1):63-72. doi: 10.1016/0165-5728(93)90285-7.

DOI:10.1016/0165-5728(93)90285-7

PMID:8376549

Abstract

Anti-normal brain antibodies were studied in glioma cyst fluids. Cyst fluids were obtained by stereotaxic puncture from 34 patients with cystic gliomas. Immunoglobulins were analyzed by immunohistochemistry, immuno-Western blotting, concentration measures, and isoelectrofocalization. 80% of cyst fluids stained astrocytes and/or microvessels in non-tumoral white matter. In white matter extracts, cyst fluids recognized five immunoreactive bands having apparent molecular masses of 50-75 kDa. Although cyst proteins could be of systemic origin, isoelectrofocalization analysis suggests an additional local immune response. These antibody activities could be involved in certain peritumoral events such as brain edema.

摘要

对胶质瘤囊液中的抗正常脑抗体进行了研究。通过立体定向穿刺从34例囊性胶质瘤患者获取囊液。采用免疫组织化学、免疫蛋白质印迹法、浓度测定及等电聚焦法分析免疫球蛋白。80%的囊液使非肿瘤性白质中的星形胶质细胞和/或微血管着色。在白质提取物中，囊液识别出5条免疫反应带，表观分子量为50 - 75 kDa。尽管囊蛋白可能来源于全身，但等电聚焦分析提示存在额外的局部免疫反应。这些抗体活性可能参与某些瘤周事件，如脑水肿。