Birkmayer G D, Stass H P
Int J Cancer. 1980 Apr 15;25(4):445-52. doi: 10.1002/ijc.2910250404.
Plasma membranes from normal and neoplastic human brain tissue were isolated and compared morphologically, biochemically and immunologically. There ultrastructure was identical, electrophoresis on polyacrylamide gel revealed at least 24 protein components, four of which were absent from membranes of normal tissue, whereas one was missing in the tumor pattern. The tumor-associated components could be solubilized by the non-ionic detergent Triton-X100 and were used as an antigen source for the immunological characterization of glioma membranes. By means of a rabbit antiserum immunologically identical components could be detected in the Triton extract of the membranes from all the 20 human gliomas tested so far, but not in normal or fetal brain, nor in liver, kidney or spleen tissue. These data indicate that the solubilized membrane components contain common glioma-associated membrane antigens which might be used in the detection of circulating antibodies in the sera of glioma patients. Of the 24 samples of sera tested so far, 19 (79%) contained precipitating antibodies against these membrane components as revealed by countercurrent electrophoresis.
分离了来自正常和肿瘤性人脑组织的质膜，并对其进行了形态学、生物化学和免疫学比较。它们的超微结构相同，聚丙烯酰胺凝胶电泳显示至少有24种蛋白质成分，其中4种在正常组织的膜中不存在，而有一种在肿瘤模式中缺失。肿瘤相关成分可用非离子去污剂Triton-X100溶解，并用作胶质瘤膜免疫学特征分析的抗原来源。借助兔抗血清，在迄今为止测试的所有20个人类胶质瘤膜的Triton提取物中都能检测到免疫学上相同的成分，但在正常或胎儿脑、肝脏、肾脏或脾脏组织中则检测不到。这些数据表明，溶解的膜成分含有常见的胶质瘤相关膜抗原，这些抗原可用于检测胶质瘤患者血清中的循环抗体。在迄今为止测试的24份血清样本中，有19份（79%）通过对流电泳显示含有针对这些膜成分的沉淀抗体。
