Bamberger D M, Herndon B L, Suvarna P R
Section of Infectious Diseases, School of Medicine, University of Missouri-Kansas City 64108-2792.
J Infect Dis. 1993 Oct;168(4):893-6. doi: 10.1093/infdis/168.4.893.
Microbial growth and antimicrobial bacterial killing are both diminished in abscesses. It was postulated that zinc depletion in abscesses, perhaps secondary to a neutrophil protein resembling calprotectin, may be partly responsible for these effects. In a rabbit tissue-cage abscess model, pooled abscess supernatant concentration of zinc was < 1.53 microM. The addition of 41.7 microM zinc had no effect on Staphylococcus aureus growth or the bacterial killing effect of cefazolin in serum. In abscess fluid supernatants, bacterial growth without antibiotic and bacterial killing by cefazolin were both enhanced by the addition of zinc. Fractionation of the abscess fluid with ultrafiltration membranes showed that these effects could be reproduced with the fraction between 30 and 50 kDa. These findings suggest that a protein in abscess fluid supernatants that resembles the neutrophil protein calprotectin may, through its zinc binding effects, inhibit microbial growth within an abscess but also inhibit the activity of bactericidal antibiotics.
在脓肿中，微生物生长和抗菌性细菌杀灭作用均减弱。据推测，脓肿中的锌缺乏，可能继发于一种类似于钙卫蛋白的中性粒细胞蛋白，可能部分导致了这些效应。在兔组织笼脓肿模型中，脓肿上清液中锌的合并浓度<1.53微摩尔/升。添加41.7微摩尔/升的锌对金黄色葡萄球菌的生长或头孢唑林在血清中的细菌杀灭作用没有影响。在脓肿液上清液中，添加锌可增强无抗生素时的细菌生长以及头孢唑林的细菌杀灭作用。用超滤膜对脓肿液进行分级分离表明，分子量在30至50 kDa之间的级分可重现这些效应。这些发现表明，脓肿液上清液中一种类似于中性粒细胞蛋白钙卫蛋白的蛋白质，可能通过其锌结合作用，抑制脓肿内的微生物生长，但也抑制杀菌性抗生素的活性。
