Sasaguri S, Hosoda Y, Tahara M, Watanabe T, Kazui M, Kikuchi N, Goto M, Yamamuro M, Yamamoto S, Nukariya M
Department of Cardio-Thoracic Surgery, Juntendo University, Tokyo, Japan.
Nihon Kyobu Geka Gakkai Zasshi. 1993 Jul;41(7):1188-93.
The arterial conduits such as internal thoracic artery (ITA) and right gastroepiploic artery (GEA) are widely used in coronary artery bypass surgery because of their resistance to atherosclerosis. In this study, immunophenotypes of smooth muscle cells (SMCs) in intima and media of ITA, GEA and saphenous vein (SV) were studied using monoclonal antibodies specific to cytoskeletal proteins; actin (A), vimentin (V) and desmin (V). In addition, the ultrastructures of endothelium of these vessels were examined. The most SMCs in intima and media of ITA and GEA were found positive for (A) and (V) but negative for (D). In contrast, the majority of SMCs both in intima and media of SV were found positive for (A), (V) and (D). The ultrastructure of endothelium of ITA and GEA showed the deeper penetration of cytoplasmic process than SV, which might anchor the endothelium. We suggest the morphological difference of endothelium and phenotypic diversity of SMCs between arterial and venous grafts may account for the different susceptibility to atherosclerotic changes in coronary bypass grafting.
诸如胸廓内动脉（ITA）和右胃网膜动脉（GEA）等动脉血管移植物，因其对动脉粥样硬化具有抗性，而在冠状动脉旁路移植手术中被广泛应用。在本研究中，我们使用针对细胞骨架蛋白肌动蛋白（A）、波形蛋白（V）和结蛋白（D）的单克隆抗体，研究了ITA、GEA和大隐静脉（SV）内膜和中膜中平滑肌细胞（SMC）的免疫表型。此外，还检查了这些血管内皮的超微结构。结果发现，ITA和GEA内膜和中膜中的大多数SMC对（A）和（V）呈阳性反应，而对（D）呈阴性反应。相比之下，SV内膜和中膜中的大多数SMC对（A）、（V）和（D）均呈阳性反应。ITA和GEA内皮的超微结构显示，其细胞质突起的穿透深度比SV更深，这可能有助于固定内皮。我们认为，动脉移植物和静脉移植物之间内皮的形态差异以及SMC的表型多样性，可能是冠状动脉旁路移植术中移植物对动脉粥样硬化变化易感性不同的原因。
