碎米荠褪绿斑点香石竹潜隐病毒的基因组序列。

The genomic sequence of cardamine chlorotic fleck carmovirus.

作者信息

Skotnicki M L, Mackenzie A M, Torronen M, Gibbs A J

机构信息

Research School of Biological Sciences, Australian National University, Canberra, A.C.T.

出版信息

J Gen Virol. 1993 Sep;74 ( Pt 9):1933-7. doi: 10.1099/0022-1317-74-9-1933.

DOI:10.1099/0022-1317-74-9-1933

PMID:8376969

Abstract

The complete genomic sequence of cardamine chlorotic fleck carmovirus (CCFV) has been determined. The genome is a positive-sense ssRNA molecule 4041 nucleotides in length, and has 47 to 64% sequence identity with turnip crinkle, carnation mottle and melon necrotic spot carmoviruses. CCFV and these other carmoviruses have four similar open reading frames (ORFs), and CCFV has large regions of amino acid identity in all of these ORFs with a European isolate of turnip crinkle virus. CCFV, which replicates well in Arabidopsis thaliana, has only been found so far in Australia in the wild perennial brassica Cardamine lilacina.

摘要

已确定碎米荠褪绿斑点香石竹潜隐病毒（CCFV）的完整基因组序列。该基因组是一个长度为4041个核苷酸的正义单链RNA分子，与芜菁皱缩病毒、香石竹斑驳病毒和甜瓜坏死斑点香石竹潜隐病毒具有47%至64%的序列同一性。CCFV和其他这些香石竹潜隐病毒具有四个相似的开放阅读框（ORF），并且CCFV在所有这些ORF中与欧洲分离的芜菁皱缩病毒具有大片段的氨基酸同一性。CCFV在拟南芥中复制良好，迄今为止仅在澳大利亚的野生多年生十字花科碎米荠中发现。